Das MP-SPR Navi™ 420A ILVES (benannt nach dem finnischen Luchs) ist die ideale Lösung für molekulare Interaktionsstudien mit MP-SPR im Hochdurchsatz. Minimale Totvolumina in der 4-Kanal-Fluidik machen es auch zur ersten Wahl bei der Untersuchung kleiner Moleküle.
Dafür kommt das ILVES standardmäßig mit zwei Lasern für jeden Kanal, die übrigens parallel für hohen Durchsatz oder seriell für differenzierte Analysen schaltbar sind. Die KineticTitration bietet zusätzliche Geschwindigkeit, und das enthaltene TraceDrawer Softwarepaket die entsprechende Auswertung der erhobenen Daten.
MP-SPR Navi 420A ILVES
- Goniometrische SPR-Optik mit Breitwinkel-Kurvenaufnahme
- Optimiert für Bindungsanalysen im Hochdurchsatz
- Automatische Probenzuführung aus 96well oder 384well Mikrotiterplatten
- 4 Messkanäle mit paralleler oder serieller Injektion
- 1-2 Laser
- integrierte Entgasungseinheit
- Software: NaviTM-Control und DataViewer enthalten, mit PureKineticsTM-Funktionalität
- KinetricTitration für schnellere Affinitäts- und Kinetikanalysen
- TraceDrawer Software enthalten, LayerSolver optional
- diverse Flusszellen verfügbar, e.g. für LS, MS, Elektrochemie, Lichteinkopplung, Fluoreszenzeinkopplung
Für die vielen Anwendungen der MP-SPR ...
... und umfangreiche Detailinformationen sowie eine Stichwortsuche nutzen Sie bitte hier unten die Links auf die Herstellerseite von BioNavis.
Pharmaceuticals |
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With PureKinetics™ feature for high quality kinetics, MP-SPR is a powerful and sensitive tool for direct measurements of small molecules. |
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MP-SPR helps you to measure biomembrane interaction kinetics as well as underlying structural changes. |
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Whether you develop metal, silica, polymer nanoparticles or work with DNA polyplexes, liposomes, viruses or microvesicles, MP-SPR is an excellent choice for drug delivery studies - from real-time targeting studies to real-time internalization with the same instrument. |
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MP-SPR enables to move from drug-target measurements, through drug-membrane interactions all the way to drug-cell interactions. Differentiate internalization from permeation in real-time and label-free. Study cell attachment on different coatings. |
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Antibody-antigen interaction affinity and kinetic measurements can be performed in diverse liquids including complex liquids such as serum or saliva.
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Biosensor development |
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Electrochemistry |
MP-SPR can be combined with electrochemistry to measure catalytic interactions, electroswitching surfaces and also to develop electrochemical biosensors. |
| Biosensor Development |
From nanoparticle-based competitive assays through electrochemical sensors to direct assays, MP-SPR shows all the steps of your assay may you develop it on top of glass, polymer, silica or metal surfaces! More... |
| Gas Sensor Development | MP-SPR works also with gas samples - from 2 dalton hydrogen up to vapours. More.. |
Biomaterials and coatings |
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| Biomaterials | MP-SPR measures interactions on polymers up to 5 µm thick, which makes it the most sensitive label-free technique for biomaterial interaction studies and layer characterization. Therefore, MP-SPR also helps with formulations for controlled drug release, novel coatings for cell and tissue engineering as well as industrial coatings. |
| Biocompatibility | Unique Multi-Parametric Surface Plasmon Resonance (MP-SPR) instruments can extend applicability of SPR also to clinically relevant complex liquids, such as 100 % serum, plasma, urine, bacteria and cells. |
420A ILVES
| Measurement principle |
Real-time and label-free Multi-Parametric Surface Plasmon Resonance (MP-SPR) is based on a true goniometric SPR arrangement with a rotating detector. Exceptionally wide angular range 40-78 ̊ is measured, real angular resolution 0.001 ̊. |
| Liquid handling |
Automated Liquid handling from 96well plate for unattended runs. Precise syringe pumps and integrated degasser. Flow rate from 1 μl/min up to 1000 μl/min. 4 flow channels, software switch for serial or parallel injection. Dual loop injection mode for KineticTitration functionality. |
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Sample consumption |
Minimum sample loop volume 100 µl, partial loop injection option, minimum injection volume 50µl.. PureSampleTM function. |
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Wavelength of light |
Dual wavelength, 670nm and 785nm in all flow channels. |
| Refractive index range |
1.00-1.40 (measurement bulk environment) which can be extended with additional wavelength |
| Media |
Measurements can occur in gas and liquids. Liquids can be aqueous solutions or organic liquids such as ethanol and acetonitrile or DMSO. Or complex biological matrices (serum, saliva, cell culture media). |
| Mode of operation |
Angular Scanning mode, or “MP-SPR mode”: scanning across a range of angles providing full SPR curve and multiple parameters. Several sensograms can be distinguished from the full curve, such as PureKineticsTM. Sampling rate depends on selected angular range and resolution, typically less than 2 seconds. Fixed Angle mode, “traditional SPR mode”: measurement at a single angle, providing time – intensity sensogram. Mode for fast kinetic studies - sampling rate from 1ms |
| Measurement Range |
Thickness from Ångströms to micrometers (true range depends on refractive index of the material). Kinetics: ka = 103 – 108 1/(M*s), kd = 10-7 – 0.1 1/s, KD = 10-3 – 10-12 M |
| Sensitivity |
Smallest detected molecule: in gas Hydrogen (2 Da); in liquid small molecules <100 Daltons |
| Noise |
Short-term noise 0.3 μRIU, Baseline drift (long term) <1μRIU/min. |
| Temperature | Measurement temperature range from 15 – 45 ̊C (7 ̊ below to 20 ̊C above ambient). |
| Prism | Elastomer supported prism for quick sensor exchange and easy maintenance |
| Flow Cells |
Flow cells can be easily exchanged with a single release button. |
| Software | Unlimited MP-SPR NaviTM Control and DataViewer software. Export data easily to Excel or use our dedicated analyzing tools. Optional LayerSolverTM fitting tool for layer characterization and/ or TraceDrawerTM software for kinetic analysis. |
| Maintenance |
Fluidic parts are easily exchanged. Maintenance service contract available if so desired. |
| Computer Requirements | Win 7, Win 8.1 or Win 10, 1 x USB 2.0, 4GB RAM, 10GB hard disk space (1GB for installation + space for measured data) |
| Dimensions & Weight |
W 62 x H 41 x D 47 cm (24” x 16” x 18,5”), 50 kg (48 lbs) |
| Power Requirements | 100-240V, 50/60Hz, Max. 100W |