The MP-SPR NaviTM 400 KONTIO (named after the Finnish bear) is specifically designed for working with cells, e.g. cell adhesion and cell-based interactions under physiologically relevant conditions. The instrument can be used with complex cell culture media and other matrices such as serum or saliva.
Its 4-fluidic channels on the sensor, which can be switched in parallel or series, offer interesting experimental options, and the optional additional wavelength offers insights into the layer structure on the sensor surface.
Various flow cells are available for the different application areas. The open design and the resulting easy maintenance makes it a very cost-effective 4-channel instrument with enormous experimental possibilities, even in the long run.
MP-SPR Navi 400 KONTIO
- Goniometric SPR optics with wide-angle curve acquisition
- Manual sample feeding
- 4 measuring channels with parallel or serial injection
- 1-2 lasers
- optional degassing unit
- Software: NaviTM Control and DataViewer included,
- with PureKineticsTM functionality
- LayerSolver and TraceDrawer optional
- various flow cells available, e.g. for LS, MS, electrochemistry, light coupling, fluorescence coupling
For the many applications of the MP-SPR ...
... and extensive detailed information as well as a keyword search, please use the links to the manufacturer's page of BioNavis here below.
Pharmaceuticals |
|
|
|
With PureKinetics™ feature for high quality kinetics, MP-SPR is a powerful and sensitive tool for direct measurements of small molecules. |
|
|
MP-SPR helps you to measure biomembrane interaction kinetics as well as underlying structural changes. |
|
|
Whether you develop metal, silica, polymer nanoparticles or work with DNA polyplexes, liposomes, viruses or microvesicles, MP-SPR is an excellent choice for drug delivery studies - from real-time targeting studies to real-time internalization with the same instrument. |
|
|
MP-SPR enables to move from drug-target measurements, through drug-membrane interactions all the way to drug-cell interactions. Differentiate internalization from permeation in real-time and label-free. Study cell attachment on different coatings. |
|
|
Antibody-antigen interaction affinity and kinetic measurements can be performed in diverse liquids including complex liquids such as serum or saliva.
|
Biosensor development |
|
|
Electrochemistry |
MP-SPR can be combined with electrochemistry to measure catalytic interactions, electroswitching surfaces and also to develop electrochemical biosensors. |
| Biosensor Development |
From nanoparticle-based competitive assays through electrochemical sensors to direct assays, MP-SPR shows all the steps of your assay may you develop it on top of glass, polymer, silica or metal surfaces! More... |
| Gas sensor development | MP-SPR works also with gas samples - from 2 dalton hydrogen up to vapours. More.. |
Biomaterials and coatings |
|
| Biomaterials | MP-SPR measures interactions on polymers up to 5 µm thick, which makes it the most sensitive label-free technique for biomaterial interaction studies and layer characterization. Therefore, MP-SPR also helps with formulations for controlled drug release, novel coatings for cell and tissue engineering as well as industrial coatings. |
| Biocompatibility | Unique Multi-Parametric Surface Plasmon Resonance (MP-SPR) instruments can extend applicability of SPR also to clinically relevant complex liquids, such as 100 % serum, plasma, urine, bacteria and cells. |
400 Kontio
| Measurement principle |
Real-time and label-free Multi-Parametric Surface Plasmon Resonance (MP-SPR) is based on a true goniometric SPR arrangement with a rotating detector. Exceptionally wide angular range 40-78 ̊ is measured, real angular resolution 0.001 ̊. |
| Liquid handling |
Controlled buffer flow conditions with 2 peristaltic pumps and 2 flow channels. Flow rate range from 10 μl/min up to 400 μl/min. 4 flow channels and manual sample injector. Instrument can be later upgraded with dedicated autosampler to a 410A KAURIS. |
|
Sample consumption |
Typical required sample volume 200-500 μl. Sample volume can be further varied by changing sample loops. |
|
Wavelength of light |
Standard 670 nm in both flow channels. |
| Refractive index range |
1.00-1.40 (measurement bulk environment) which can be extended with additional wavelength |
| Media |
Measurements can occur in gas and liquids. Liquids can be aqueous solutions or organic liquids such as ethanol and acetonitrile. |
| Mode of operation |
Angular Scanning mode, or “MP-SPR mode”: scanning across a range of angles providing full SPR curve and multiple parameters. Several sensograms can be distinguished from the full curve, such as PureKineticsTM. Sampling rate depends on selected angular range and resolution, typically less than 2 seconds. Fixed Angle mode, “traditional SPR mode”: measurement at a single angle, providing time – intensity sensogram. Mode for fast kinetic studies - sampling rate from 1ms |
| Measurement Range |
Thickness from Ångströms to micrometers (true range depends on refractive index of the material). Kinetics: ka = 103 – 108 1/(M*s), kd = 10-7 – 0.1 1/s, KD = 10-3 – 10-12 M |
| Sensitivity |
Smallest detected molecule: in gas Hydrogen (2 Da); in liquid small molecules <100 Daltons |
| Noise |
Short-term noise 0.3 μRIU, Baseline drift (long term) < 1μRIU/min. |
| Temperature | Measurement temperature range from 15 – 45 ̊C (7 ̊ below to 20 ̊C above ambient). |
| Prism | Elastomer supported prism for quick sensor exchange and easy maintenance |
| Flow Cells |
Flow cells can be easily exchanged with a single release button. |
| Software | Unlimited MP-SPR NaviTM Control and DataViewer software. Export data easily to Excel or use our dedicated analyzing tools. Optional LayerSolverTM fitting tool for layer characterization and/ or TraceDrawerTM software for kinetic analysis. |
| Maintenance |
Fluidic parts are easily exchanged. Maintenance service contract available if so desired. |
| Computer Requirements | Win 7, Win 8.1 or Win 10, 1 x USB 2.0, 4GB RAM, 10GB hard disk space (1GB for installation + space for measured data) |
| Dimensions & Weight |
W 33 x H 42 x D 39 cm (13” x 17” x 15”), 11 kg (24 lbs) |
| Power Requirements | 100-240V, 50/60Hz, Max. 40W |